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Chromatographic conditions employ a C18 reversed-phase column, with acetonitrilewater solution (adjusted to an appropriate acidity with phosphoric acid) as the mobile phase

These amenable properties demonstrated for the small molecules led us to conduct a proof-of-concept in vivo study in diet-induced obese mice to test the hypothesis that the most potent inhibitor when administered systemically, would reverse obesity by causing substantial loss of body weight and adiposity without causing any observable adverse effects

By doing so, it encourages fat loss while preventing muscle wasting, which is one of the key concerns when undergoing fat loss

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